Sakura made her first appearance in Trouble's Brewing. She was particularly friendly with Misty, due to having the common experience of being the youngest in a group of sisters.
In Espeon, Not Included, the group met up with Sakura again while passing through to Ecruteak City. By this time, her Eevee had evolved into an Espeon.
Sakura's most recent appearance was in the special episode The Blue Badge of Courage. She is now challenging gyms and so decided to visit Misty in Cerulean City.